Chemical composition of the lipid-protein fraction separated in the production of Epsorin biopreparation

Abstract

The chemical composition of the lipid-protein fraction separated in the production of the biological preparation Epsorin was studied. Epsorin, the drug based on reindeer antlers, is used as an effective medicine for asthenic conditions of various origins, with neurasthenia, neurosis, in postoperative periods, with weakness of the heart muscle, with hypotension; this drug has immunomodulating and radioprotective effect, stimulating and strength-recovering properties, it increases the physical and mental performance. The fractions of lipids, water-soluble substances and hydrolysis products were isolated from the lipid-protein mass by sequential extraction from the residues of the production of Epsorin biopreparation. All fractions obtained were converted to trimethylsilyl (TMS) derivatives. The obtained TMS derivatives were analyzed by means of GC-MS using a MAESTRO 7820/5975 instrument (Russia), built on the basis of an Agilent 7820 gas chromatograph (USA) and an Agilent 5975 mass spectrometric detector. It was shown that the yield of the studied substances was higher after freeze drying than after thermal treatment. A high content of replaceable and irreplaceable amino acids, polyols, organic, inorganic and fatty acids in the «Epsorin» lipid-protein residue was established. Thus, the Epsorin lipid-protein residue can be used as a biologically active cosmetic additive.