Morphogenesis of the Onobrychis Arenaria In Vitro and Its Control by Using Peat Humic Acids and Nano-Biocomposites
Abstract
The influence of a mineral composition of the nutrient solutions and new growth stimulants from a vegetable origin raw materials on the morphogenesis of Onobrychis arenaria in a culture of the tissue in vitro studied. The new growth stimulants from a vegetable origin raw materials, which were put into composition of the nutrient media for the recurrent regeneration and the micro-cloning multiplication of the onobrychis in concentration 0.5–1 mg/l, have an effect on the morphogenesis and permit to change the expensive synthetical cytokinins and the auxins, reduce the cultivation’s time in vitro in 1.5–2 times, increase the appearance of the scions and the frequency of rhizogenesis by 15–90%. The effectiveness of the antiseptic treatment of the seeds of the onobrychis with a mixture of ethanol and hydrogen dioxide achieves 97 % and the germination of the aseptic seeds under laboratory conditions is 72 %.
The protocols of the recurrent regeneration with nano-biocomposites as the alternatives of the synthetical plant hormones are worked out. The new selection biological technologies are applicable for creation of highly productive and longeval forms of onobrychis, which are resistent in conditions of the cryolithic zone.
About the Authors
Olga Aleksandrovna Rozhanskaya
Scientific Research Institute of Fodder SB RAS
Russian Federation
Russian Federation
Nataliya Vladimirovna Barashkova
Institute for Biological Problems of Cryolithozone SB RAS
Russian Federation
Russian Federation
Tatyana Vasilievna Shilova
Scientific Research Institute of Fodder SB RAS
Russian Federation
Russian Federation
Valentina Gavril’evna Darkhanova
Institute for Biological Problems of Cryolithozone SB RAS
Russian Federation
Russian Federation
Nataliya Semenovna Stroeva
Institute for Biological Problems of Cryolithozone SB RAS
Russian Federation
Russian Federation
References
1. Черепанов С.К. Сосудистые растения СССР. – Л.: Наука, 1981.– 510 с.
2. Рожанская О.А. Создание исходного материала для селекции кормовых культур в условиях Сибири с помощью методов биотехнологии: Автореф. дис. … д.б.н. – СПб., 2007. – 33 с.
3. Рожанская О.А., Дарханова В.Г., Строева Н.С. Aвтоселекция in vitro эспарцета песчаного на адаптивность // Сиб. вест. с-х. науки. – 2011. – №3–4. – С. 131–134.
4. Рожанская О.А., Дарханова В.Г., Строева Н.С. и др. Особенности регуляции морфогенеза эспарцета и люцерны in vitro // Сиб. вест. с-х. науки. – 2008. –
5. №5. – С. 58–65.
6. Gamborg O.L. Nutrient requirement of suspension cultures of soybean root cell / O.L. Gamborg, R.A. Miller, K. Ojima // Exp.Cell Res. – 1968. – V. 50. – P. 151–158.
7. Murashige T. A revised medium for rapid growth and bio assays with tobacco tissue cultures / T. Murashige, F. Skoog // Physiol. plant. – 1962. – V. 15. – P. 473–497.
8. Рожанская О.А., Инишева Л.И., Ларина Г.В. Характеристика биологической активности препаратов гуминовых кислот торфов в культуре тканей растений // Сиб. вест. с-х. науки. – 2013. – №3. – С. 20–27.
9. Korolev K.G., Lomovskij O.I., Rozhanskaya O.A., Vasil'ev V.G. Mechanochemical preparation of water-soluble forms of triterpene acids // Chem. Natural compounds. – 2003. – 39:4. – P. 366–372.
10. Патент РФ № 2267927. Биостимулятор роста растений / К.Г. Королев, О.И. Ломовский, О.А. Рожанская. – Опубл. в Б.И. – 2006. – № 2.
11. Дарханова В.Г., Строева Н.С., Королев К.Г. и др. Применение нанобиокомпозитов для стимуляции роста растений in vitro / Материалы II Международного форума по нанотехнологиям (Москва, 6–8 октября 2009 г.) http://rusnanotech09.rusnanoforum.ru/cgi-bin/ show.pl?option=&id=&lang=ru.
12. Сорокин О. Д. Прикладная статистика на компьютере. – Краснообск: ГУП РПО СО РАСХН, 2004. – 162 с.
Review
For citations:
Rozhanskaya O.A., Barashkova N.V., Shilova T.V., Darkhanova V.G., Stroeva N.S. Morphogenesis of the Onobrychis Arenaria In Vitro and Its Control by Using Peat Humic Acids and Nano-Biocomposites. Arctic and Subarctic Natural Resources. 2015;20(4):111-114. (In Russ.)
Views:
10
Email this article 