Перспективность рационального использования биологически активных веществ из хвои Pinus sylvestris для создания биопрепаратов

Проведено исследование годовой динамики накопления метаболитов в хвое обыкновенной сосны (Pinus sylvestris L.), произрастающей на территории Центральной Якутии. Установлено, что в хвое сосны содержатся ценные биоактивные вещества, эффективные при профилактике и купировании нарушений обмена веществ, возникающих при сахарном диабете 2-го типа и при формировании других метаболических нарушений, связанных с гиперхолестеринемией, а также вещества криопротекторного действия. Показано, что наибольшие содержания антиоксидантов, таких как галловая, бензойная, аминомасляная кислота, наблюдаются осенью, также в этот период возрастает содержание полиолов, аминокислот и углеводов. На основании полученных данных выдвинуто предположение о том, что в целях создания биопрепаратов, нормализующих углеводный обмен, а также защищающих кожные покровы от действия низких температур, рационально использовать в качестве сырья хвою P. sylvestris, отобранную осенью.

1. Schultz D., Campeau L.-C. Harder, better, faster. Nat. Chem. 2020; 12(8):661–664. https://doi.org/10.1038/s41557-020-0510-8

2. Hughes R.C. Pricing Medicine Fairly. Philosophy of Management. 2020;19(4):369–385. https://doi.org/10.1007/s40926-020-00135-z

3. Khlebnyy E.S., Kerschengoltz B.M. Structural and functional variety of physiologically active agents – A molecular basis of high adaptive potential and a specific variety of a biota in the Arctic, prospects for biopharmaceutics. J. Ecosys. Ecograph. 2013;03(04):50. https://doi.org/10.4172/2157-7625.S1.012

4. Gromek K., Drumond N., Simas P. Pharmacovigilance of herbal medicines. JRS.2015;27(2):55–65. https://doi.org/10.3233/JRS-150643

5. Rowin J., Lewis S.L. Spontaneous bilateral subdural hematomas associated with chronic Ginkgo biloba ingestion. Neurology. 1996;46(6):1775–1776. https://doi.org/10.1212/WNL.46.6.1775

6. Guo X., Mei N. Aloe vera: A review of toxicity and adverse clinical effects. Journal of Environmental Science and Health, Part C. 2016;34(2):77–96. https://doi.org/10.1080/10590501.2016.1166826

7. Kaur S., Das M. Functional foods: An overview. Food Science and Biotechnology. Springer, 2011:20(4): 861–875. https://doi.org/10.1007/s10068-011-0121-7

8. Kato-Noguchi H., Fushimi Y., Shigemori H. An allelopathic substance in red pine needles (Pinus densiflora). Journal of Plant Physiology. 2009;166(4): 442–446. https://doi.org/10.1016/j.jplph.2008.06.012

9. Farjon A. World checklist and bibliography of conifers. Royal Botanic Gardens, 2001. 309 p.

10. Dziedziński M., Kobus-Cisowska J., Stachowiak B. Pinus species as prospective reserves of bioactive compounds with potential use in functional food – Current state of knowledge. Plants. 2021;10(7):1306. https://doi.org/10.3390/plants10071306

11. Durrant T.H., De Rigo D., Caudullo G. Pinus sylvestris in Europe: distribution, habitat, usage and threats. European atlas of forest tree species. Publ. Off. EU Luxembourg, 2016:132–133.

12. Nikolaev A.N., Fedorov P.P., Desyatkin A.R. Effect of hydrothermal conditions of permafrost soil on radial growth of larch and pine in Central Yakutia. Contemp. Probl. Ecol. 2011:4(2):140–149. https://doi.org/10.1134/S1995425511020044

13. Sudachkova N. Ye. et al. Physiology of the common pine. Novosibirsk: Nauka; 1990. 244p. (In Russ.)

14. Egorov A.D. Vitamin C and carotene in the vegetation of Yakutia. M.: Izd-vo AN SSSR; 1954. 246 p. (In Russ.)

15. Hoai N. et al. Selectivity of Pinus sylvestris extract and essential oil to estrogen-insensitive breast cancer cells Pinus sylvestris against cancer cells. Phcog Mag. 2015;11(44):290. https://doi.org/10.4103/0973-1296.166052

16. Nikonova N.N. et al. “Green technology” processing of pine (Pinus sylvestris L.) and larch (Larix sibirica Ledeb.) wood greenery to produce bioactive extracts. Holzforschung. 2022;76(3):276–284. https://doi.org/10.1515/hf-2021-0122

17. Strizincova P., Jablonsky M., Lelovsky M. Bioactive compounds of softwood bark as potential agents against human diseases include the SARS-CoV-2 virus. Biointerface Res. Appl. Chem. 2021;12(5):5860–5869. https://doi.org/10.33263/BRIAC125.58605869

18. Bibik I.V., Glineva Yu.A. Prospects of using pine needles extract in the production of functional drinks. Food processing: techniques and technology. 2012;1(24): 9–13. (In Russ.)

19. Zhuravleva L.N., Devyatlovskaya A.N., Rubchevskaya L.P. The woody greens of scots pine as a promising source for biologically active substances. Bulliten KrasSAU. 2008;(3):166–169. (In Russ.)

20. Слепцов И.В., Рожина С.М. Эколого-географические особенности накопления метаболитов в хвое Larix cajanderi на территории Якутии. Химия растительного сырья. 2021;(2):275–280. https://doi.org/10.14258/jcprm.2021028322

21. Konoreva L. et al. Metabolite profiling of the Cladonia lichens using gas chromatography-mass spectrometry. Biochemical Systematics and Ecology. 2019;85: 3–12. https://doi.org/10.1016/j.bse.2019.04.004

22. Gao Y. et al. Effects of D-pinitol on insulin resistance through the PI3K/Akt signaling pathway in Type 2 diabetes mellitus rats. J. Agric. Food Chem. 2015;63(26): 6019–6026. https://doi.org/10.1021/acs.jafc.5b01238

23. Lee E. et al. Pinitol consumption improves liver health status by reducing oxidative stress and fatty acid accumulation in subjects with non-alcoholic fatty liver disease: A randomized, double-blind, placebo-controlled trial. The Journal of Nutritional Biochemistry. 2019; 68:33–41. https://doi.org/10.1016/j.jnutbio.2019.03.006

24. Zheng K. et al. Protective effect of pinitol against inflammatory mediators of rheumatoid arthritis via inhibition of protein tyrosine phosphatase non-receptor Type 22 (PTPN22). Med. Sci. Monit. 2017;23:1923–1932. https://doi.org/10.12659/MSM.903357

25. Lin T.-H. et al. D-pinitol inhibits prostate cancer metastasis through inhibition of αVβ3 integrin by modulating FAK, c-Src and NF-κB pathways. IJMS. 2013; 14(5):9790–9802. https://doi.org/10.3390/ijms14059790

26. Kim J.-I. et al. Effects of pinitol isolated from soybeans on glycaemic control and cardiovascular risk factors in Korean patients with type II diabetes mellitus: a randomized controlled study. Eur. J. Clin. Nutr. 2005; 59(3):456–458. https://doi.org/10.1038/sj.ejcn.1602081

27. Pintaudi B., Di Vieste G., Bonomo M. The effectiveness of myo-inositol and D-chiro-inositol treatment in Type 2 diabetes. International Journal of Endocrinology. 2016;2016:1–5. https://doi.org/10.1155/2016/9132052

28. Jariwalla R.J. Inositol hexaphosphate (IP6) as an anti-neoplastic and lipid-lowering agent. Anticancer Res. 1999;19(5A):3699–3702.

29. Xu Y. et al. Gallic Acid and Diabetes Mellitus: Its association with oxidative stress. Molecules. 2021.26(23): 7115. https://doi.org/10.3390/molecules26237115

30. Aglan H.A. et al. Gallic acid against hepatocellular carcinoma: An integrated scheme of the potential mechanisms of action from in vivo study. Tumour Biol. 2017;39(6):101042831769912. https://doi.org/10.1177/1010428317699127

31. Orthen B., Popp M. Cyclitols as cryoprotectants for spinach and chickpea thylakoids. Environmental and Experimental Botany. 2000;44(2):125–132. https://doi.org/10.1016/S0098-8472(00)00061-7

32. Fischer C., Höll W. Food reserves of Scots pine (Pinus sylvestris L.).Trees. 1991;5(4):187–195.

33. Алексеев Р.З. и др. Предупреждение развития некроза при отморожениях с оледенением тканей. Международный журнал прикладных и фундаментальных исследований. 2015;8(1):35–41.

34. Gupta A., Soni R., Ganguli M. Frostbite – manifestation and mitigation. Burns Open. 2021;5(3):96–103. https://doi.org/10.1016/j.burnso.2021.04.002

35. Lehmuskallio E. Emollients in the prevention of frostbite. International Journal of Circumpolar Health. 2000.59(2):122–130.

36. Heisig M. et al. Frostbite Protection in mice expressing an antifreeze glycoprotein. PLoS ONE / ed. Bergmann A. 2015;10(2):e0116562. https://doi.org/10.1371/journal.pone.0116562

37. Sun M.-L. et al. Promotion of wound healing and prevention of frostbite injury in rat skin by exopolysaccharide from the arctic marine bacterium Polaribacter sp. SM1127. Marine Drugs. 2020;18(1):48. https://doi.org/10.3390/md18010048

38. Патент 2678188 C1 Российская Федерация, МПК A61K 9/10, A61K 35/64, A61K 31/045. Средство защиты кожи от холодового повреждения / Ли Н.Г., Осаковский В.Л., Осаковский А.В. Заявитель ООО «Криопротект», заявл. 31.08.2018; опубл. 24.01.2019.

39. Vinokurov M., Tikhonov D. Is the increase in the incidence of Type 2 diabetes in Yakutia due to a decrease in cold exposure or dietary changes? Siberian Research. 2022;7(1):33–37. https://doi.org/10.33384/26587270.2022.07.01.06e