In vitro Callus Induction and Plant Regeneration from Leaf Explants of Medicago varia
Abstract
The features of callus formation in vitro from leaf explants of Medicago varia Syulinskaya variety are investigated and conditions for plant regeneration from embryogenic callus tissue are tested. The induction of callus cells from leaf explants was carried out in the presence of a nutrient medium D 2,4 dichlorophenoxyacetic acid (2,4 D), kinetin and naphthaleneacetic acid (NAA). Somatic cells differentiated to embryonic in the presence of a high concentration of auxin 8 mg/l 2,4 D and kinetin; NAA 5 mg/l in the modified Gamborg B5 medium. Callus formed along the incisions and gradually covered the entire surface of the explant area. It had a yellow color, dense texture and grain structure. Passaging the derived callus on fresh agar Gamborg B5 medium (10 g/l sucrose + 4 g/l of bactoagar supplemented with BAP (0.2 mg/l), stimulated the development into callus tissue cells of meristematic and embryonic types which formed the buds and embryoids. The regenerated plants of M. varia from embryoids and buds are obtained for further development and rooting.
Keywords
Medicago varia,
auxins,
2,
4-D,
NAA,
cytokinins,
BAP,
kinetin,
plant hormones,
callusogenesis,
callus tissue,
morphogenesis,
somatic cells,
embryoids,
regenerated plants
About the Authors
Nataliya Semenovna Stroeva
Institute for Biological Problems of Cryolithozone
Russian Federation
Russian Federation
Valentina Gavril’evna Darhanova
Institute for Biological Problems of Cryolithozone
Russian Federation
Russian Federation
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Review
For citations:
Stroeva N.S., Darhanova V.G. In vitro Callus Induction and Plant Regeneration from Leaf Explants of Medicago varia. Arctic and Subarctic Natural Resources. 2017;22(1):114-116. (In Russ.)
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