The content of flavonoids in Atriplex patula L. biomass during mechanical activation treatment
https://doi.org/10.31242/2618-9712-2020-25-3-8
Abstract
The effect of grinding of the aboveground biomass of Atriplex patula L. to particle size of 1.0–1.5 mm (coarse grinding), 0.8–0.9 mm (average grinding) and 0.5–0.6 mm (fine grinding) with further processing in a mechanical mill on the total content of flavonoids, amounts of rutin, dihydroquercetin and luteolin-7-o-glucoside in vegetable flour was studied. The total content of flavonoids in the biomass was determined by means of spectrophotometry, the content of rutin, dihydroquercetin and luteolin-7-O-glucoside was determined by means of HPLC. It was found that the yield of the total amount of flavonoids increased by a factor of 1.2 when crushed to a particle size of 0.5–0.6 mm, with respect to the studied grinding versions. Mechanical activation (MA) for one minute led to a decrease in the total amount of flavonoids in all grinding versions. It was found that grinding to a particle size of 0.8–0.9 mm (average grinding) reduces the content of rutin in plant flour by a factor of 1.6–2.0 with respect to the control. MA of coarse flour increased the yield of rutin, with the exception of the two-minute MA treatment. It was found that grinding to the particle size of 0.5-0.6 mm leads to an increase in the amount of extracted dihydroquercetin; with MA treatment of the resulting flour, dihydroquercetin content does not change, except for one-minute МA, when there was a 1.8 times decrease in dihydroquercetin content. It is shown that the grinding of raw materials to the size of 1.0-1.5 and 0.5–0.6 mm causes an increase in the content of luteolin-7-O-glucoside in plant flour. The MA treatment of plant flour led to a decrease in the amount of luteolin-7-O-glucoside under these grinding conditions. The exception was MA treatment of average-milled flour, where the content of dihydroquercetin increased by 33.3 % (with respect to the control in the group). The most optimal mode of MA was established, with a rotor speed of 1500 rpm in air, with pre-MA grinding of A. patula biomass to a particle size of 0.5–0.6 mm, which does not affect the change in the amount of the studied physiologically active substances in the raw material.
Supporting agencies: This work was carried out as part of the state tasks of the IBPC SB RAS on projects № AAAA-A17-117020110056-0.
About the Author
I. V. Voronov
Institute for Biological Problems of Cryolithozone SB RAS
Russian Federation
Russian Federation
VORONOV Ivan Vasilievich, candidate of biological sciences, senior researcher
41 Lenina pr., Yakutsk 677980
Researcher ID:S-8071-2018
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Review
For citations:
Voronov I.V. The content of flavonoids in Atriplex patula L. biomass during mechanical activation treatment. Arctic and Subarctic Natural Resources. 2020;25(3):89-96. (In Russ.) https://doi.org/10.31242/2618-9712-2020-25-3-8
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