Introduction to culture and in vitro reproduction of the Hemerocallis, variety ʻWine and Rosesʼ
https://doi.org/10.31242/2618-9712-2020-25-1-9
Abstract
The possibility to use the methods and approaches of higher plant biotechnology for in vitro clonal micropropagation of the genus Hemerocallis (Krasodnev) of the varieties ʻWine and Rosesʼ was investigated. The use of generative organs (flower buds) as primary explants allowed them to be introduced into an in vitro culture without any harm to donor plants grown in the nursery. The specific features of the induction of callus formation on the nutrient medium of Murashige and Skoog in the presence of naphthylacetic acid and/or 6-benzylaminopurine are determined. The conditions for obtaining regenerated plants from embryogenic callus cultures were tested. The use of a biocomposite obtained as a growth regulator by means of mechanochemical treatment of the biological product from fir branches and needles (100 mg/l) containing water-soluble salts of triterpene acids revealed its high efficiency in the processes of morphogenesis and regeneration in the callus culture of Hemerocallis in vitro. During the three passages of callus, the development of meristematic and embryonic cells was noted, from which the kidneys and embryoids formed as a result of repeated divisions. Further cultivation led to the formation of regenerated plants. The study allowed us to obtain a sterile culture and select the composition of a nutrient medium for the successful propagation of Hemerocallis in vitro. In vitro clonal micropropagation protocol was developed for the genus Hemerocallis of the ʻWine and Rosesʼ variety, which can be an effective method for successful reproduction.
Supporting agencies: The work was carried out as part of the state assignment of the Institute of Biological Problems of the Cryolithozone of the SB RAS for 2017-2020. according to project VI.52.1.8. Fundamental and applied aspects of the study of plant diversity in Northern and Central Yakutia No. AAAA-A17-117020110056-0
About the Authors
N. S. Stroeva
Institute of Biological Problems of Cryolithozon, SB RAS
Russian Federation
Russian Federation
STROEVA Natalya Semenovna, research engineer
41 Lenin ave., Yakutsk, 677980
V. G. Darkhanova
Institute of Biological Problems of Cryolithozon, SB RAS
Russian Federation
Russian Federation
DARKHANOVA Valentina Gavril’evna, research engineer
41 Lenin ave., Yakutsk, 677980
G. V. Filippova
Institute of Biological Problems of Cryolithozon, SB RAS
Russian Federation
Russian Federation
FILIPPOVA Galina Valer’evna, senior researcher
41 Lenin ave., Yakutsk, 677980
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For citations:
Stroeva N.S., Darkhanova V.G., Filippova G.V. Introduction to culture and in vitro reproduction of the Hemerocallis, variety ʻWine and Rosesʼ. Arctic and Subarctic Natural Resources. 2020;25(1):85-93. (In Russ.) https://doi.org/10.31242/2618-9712-2020-25-1-9
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