The growth of potato micro-stalks in vitro under the action of multiconversion biopreparation Embiota
https://doi.org/10.31242/2618-9712-2021-26-3-112-119
Abstract
Results of the investigation into the effect of multiconversion biopreparation Embiota, obtained using five producing strains grown on the exhausted straw substrate of the oyster mushroom, on in vitro growth and development of micro-stalks of regenerant potato plants of «Rozara» are presented. The biopreparation was introduced in the concentrations of 2.5; 5; 10; 25; 50 and 100 mL/L into the growth medium containing the elements according to Murashige and Skoog formulation, with the full and half salt compositions. It is demonstrated that in the case of the availability of mineral nutrition, the biopreparation had no effect on the morphological parameters of vegetative organs within the whole range of studied concentrations. At the same time, in the version with 2.5 mL/L, an increase in the air-dry mass of above-ground organs by a factor of 1.3–1.4 was detected, while in the version with 100 mL/L this parameter exhibited a decrease by a factor of 1.2–1.3 in comparison with the reference. Under the conditions of insufficient nutrition, the growth-stimulating effect was registered in the versions with 5 and 10 mL/L. An increase in the parameters characterizing the number of shoots and leaves by a factor of 1.3–1.5 over the reference was detected. The concentration of 10 mL/L also has the best effect on the formation of the root system. Inhibiting action on the morphometric parameters of plants and their phytomass was detected after the introduction of the biopreparation in high concentrations 50 and/or 100 mL/L, which was especially clearly shown in the case when nutrition was insufficient. So, the results of the studies showed dose-dependent effect of «Embiota» biopreparation on the growth and development of plants. From the viewpoint of application for growth stimulation and for enhancement of productivity, the concentrations 2.5–10 mL/L may be used.
Keywords
wastes from mushroom growing,
multiconversion preparation,
potato microstalks,
in vitro,
morphometric parameters,
phytomass
Supporting agencies: The research was carried out within the State Assignment for the Institute for Biological Problems of Cryolithozone SB RAS for 2017–2020 «Fundamental and Applied Aspects of the Studies of the Diversity of the Plant Community of Northern and Central Yakutia» (No. AAAA-A17-117020110056-0).
About the Authors
G. V. Filippova
Institute for Biological Problems of Cryolithozone, SB RAS
Russian Federation
Russian Federation
FILIPPOVA, Galina Valeryevna, Cand. Sci. (Biology), senior researcher
41 Lenina st., Yakutsk 677980
V. G. Darkhanova
Institute for Biological Problems of Cryolithozone, SB RAS
Russian Federation
Russian Federation
DARKHANOVA, Valentina Gavrilyevna, research engineer
41 Lenina st., Yakutsk 677980
N. S. Stroeva
Institute for Biological Problems of Cryolithozone, SB RAS
Russian Federation
Russian Federation
STROEVA, Natalya Semenovna, research engineer
41 Lenina st., Yakutsk 677980
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For citations:
Filippova G.V., Darkhanova V.G., Stroeva N.S. The growth of potato micro-stalks in vitro under the action of multiconversion biopreparation Embiota. Arctic and Subarctic Natural Resources. 2021;26(3):112-119. (In Russ.) https://doi.org/10.31242/2618-9712-2021-26-3-112-119
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